Macrophage membrane (MMs) camouflaged near-infrared (NIR) responsive bone defect area targeting nanocarrier delivery system (BTNDS) for rapid repair: promoting osteogenesis via phototherapy and modulating immunity

Bone defects remain a significant challenge in clinical orthopedics, but no targeted medication can solve these problems. Inspired by inflammatory targeting properties of macrophages, inflammatory microenvironment of bone defects was exploited to develop a multifunctional nanocarrier capable of targeting bone defects and promoting bone regeneration. The avidin-modified black phosphorus nanosheets (BP-Avidin, BPAvi) were combined with biotin-modified Icaritin (ICT-Biotin, ICTBio) to synthesize Icaritin (ICT)-loaded black phosphorus nanosheets (BPICT). BPICT was then coated with macrophage membranes (MMs) to obtain MMs-camouflaged BPICT (M@BPICT). Herein, MMs allowed BPICT to target bone defects area, and BPICT accelerated the release of phosphate ions (PO43−) and ICT when exposed to NIR irradiation. PO43− recruited calcium ions (Ca2+) from the microenvironment to produce Ca3(PO4)2, and ICT increased the expression of osteogenesis-related proteins. Additionally, M@BPICT can decrease M1 polarization of macrophage and expression of pro-inflammatory factors to promote osteogenesis. According to the results, M@BPICT provided bone growth factor and bone repair material, modulated inflammatory microenvironment, and activated osteogenesis-related signaling pathways to promote bone regeneration. PTT could significantly enhance these effects. This strategy not only offers a solution to the challenging problem of drug-targeted delivery in bone defects but also expands the biomedical applications of MMs-camouflaged nanocarriers. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12951-024-02351-5.


Introduction
Bone defect, a disruption of the structural integrity of bone typically due to trauma, bone tumors, or osteonecrosis, is one of the main cause for non-healing or delayed bone healing [1,2].For the bone defect with critical size necessitating surgical intervention, researchers have successfully fabricated a variety of bone repair scaffolds contributed from the development of tissue engineering [3][4][5][6].However, passive self-repair is the only option for patients with non-critical-sized bone defects or patients with bone defects cannot tolerate the surgery.Notably, these patients have comprised the majority of bone defects individuals and been at a high risk of experiencing bone repair failure [7].Additionally, there are lacking clinically efficient drug delivery system for treatment of bone defect needing self-repair.Therefore, it is urgent to pay close attention to these patients' requirements for target drug delivery to the bone defect area.
Currently, researchers have designed a variety of bonetargeting nanocarrier systems (BNDS) and demonstrated that these systems are effective at promoting bone regeneration and elevating bone density [8].Although locally administration of agents is a common routine to manage the bone defect, the systematic administration might be a more appropriate method to treat non-criticalsized bone defect since it is an essentially non-invasive, dosage-regulated bone repair treatment modality.Zheng et al. conjugated alendronate with iron oxide nanoparticles to construct BNDS for treating postmenopausal osteoporosis by intravenous administration exhibiting the regulation effect on local immunity of bone tissues [9].The targeting properties of BNDS primarily stem from the strong affinity between bisphosphonates, tetracyclines, bone-targeting gene clusters, and hydroxyapatite or osteocytes in bone tissue [10][11][12].Thus, although BNDS had an affinity for bone tissues, but it was lacking specificity for bone defect area, which cannot provide adequate drugs for bone defects, ultimately leading to slow or unsuccessful repair.Additionally, BNDS is susceptible to be cleared by immune system leading to a lessened therapeutic effect.Therefore, it is imperative to design a bone defect area targeting nanocarrier delivery system (BTNDS) that can surmount these obstacles.
Previous studies have demonstrated significant microenvironmental changes in bone defect including increased infiltration of inflammatory cells, pH reduction, and a rapid elevation of Ca 2+ concentration [13].Consequently, the specific microenvironments of bone defect area might be explored to design BTNDS for the target drug delivery.The immune cells including macrophages and neutrophils or the derived cell membrane had been reported to target the inflammatory microenvironments [14].The macrophage-derived membranes (MMs) inherit surface ligands or receptors could efficiently target inflammatory sites and absorb the cytokines contributing to the reduction of inflammatory reaction [15].However, single MMs were not able to serve as an ideal vehicle for drug delivery to inflammatory areas since they were in short of long-term stability and high drug-loading efficiency.Instead, MMs could be utilized to assist other nanocarriers to target inflammatory microenvironments preventing from being cleared and exerting the absorbed performance for inflammatory factors.
Phosphorus (P) is second most abundant mineral in the body and an essential component of natural bone tissues.A two-dimensional (2D) nanomaterial, black phosphorus nanosheets (BP), has attracted tremendous attention in bone repair research due to its excellent biocompatibility and photothermal properties [16][17][18].Several studies have reported that BP could serve as drug delivery systems with ultra-high loading capacity and high-efficiency photosensitizers for photothermal therapy (PTT), which could be applied to the repair of bone defect [19,20].PTT is a non-invasive, controlled intervention strategy that can promote osteogenesis within an optimal temperature range (40-42 °C) [21,22].More importantly, BP could be continuously degraded into nontoxic PO 4 3− and capture Ca 2+ , thereby accelerating biomineralization for the bone regeneration [23,24].Shen et al. had designed a bone tissue engineering scaffold doped with BP, which exhibited a preeminent performance on the bone regeneration after combing with photothermal therapy [25].In another study, multifunctional nanospheres containing BP had been engineered for treatment of osteoporotic fracture upon near-infrared (NIR) irradiation [26].Therefore, BP is an ideal platform for delivery of bone growth agents and can target the bone defect microenvironments with the introduction of MMs.
In this study, BP had been covalently modified with avidin according to previous report [27].The avidin-coupled BP (BP Avi ) could serve as a universal and versatile platform for the efficient delivery of cargos with biotin connection.Here, Icaritin (ICT), a flavonoid monomer isolated from the Chinese herb Epimedium was taken as a modal drug after being modified with biotin.ICT had been reported to inhibit the expression of inflammatory factors and stimulate the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) [28].According to our preliminary research, ICT attached to tissue-engineered scaffolds could promote osteogenic differentiation of BMSCs by increasing the expression of bone morphogenetic protein-2 (BMP-2), Alkaline phosphatase (ALP), and Runt-related transcription factor-2 (Runx2) [29].The biotin-connected ICT could be efficiently bonded to BP Avi (BP ICT ) through non-covalent method, which would address the solubility and bioavailability issues for ICT.The MMs was introduced to prepare membrane-encapsulated BP ICT (M@ BP ICT ), which would facilitate the BP ICT with bone defect area targeting capacity.In addition, the MMs could serve as nano-sponges to absorb the inflammatory factors based on the receptors derived from macrophages.After accumulating at the bone defect, the ICT could be released from M@BP ICT exhibiting anti-inflammation and osteogenic activity.More importantly, under the irradiation of NIR, PTT could promote osteogenesis and mediate the degradation of BP into PO 4 3− to accelerate osteogenesis by capturing Ca 2+ .In short, a bone-defecttargeting nano delivery system based on bone repair material and natural compound was engineered providing a promising strategy for self-repair of non-criticalsized bone defects (Scheme 1).

BP preparation
The BP was obtained by the top-down method of liquidphase exfoliation from bulk BP.Firstly, BP bulk (500 mg) was put into DMF (30 mL).Next, the BP on the ice bath was performed with an ultrasonic probe (On/Off cycle: 5 s/10 s, Amplifier: 60%) for 8 h.Centrifugation (6000 rpm, 10 min) was used to separate large particles of BP.Afterwards, the liquid supernatant was centrifuged again at 148,000 rpm for 15 min and the precipitate of small particle size of BP was collected.

BP-avidin (BP Avi ) preparation
Avidin (1.0 mL, 0.5 mg mL −1 ) and Sulfo-SMCC (660 L, 1.0 mg mL −1 ) were dissolved in 3.34 mL of PBS.The mixture was stirred at 4 °C for 2 h before being dialyzed overnight with a dialysis bag (MWCO: 3500).The obtained product was collected by centrifugation at 14,000×g for 10 min to get maleimide-activated avidin.After dispersing BP (1.0 mg) in DMF (2.0 mL), maleimide-activated avidin (1.0 mg) in deionized water (1.0 mL) was added to the BP solution.The reaction was maintained at 30 °C for 8 h in an oil bath with magnetic stirring.The solution was then dialyzed to remove excess maleimide-activated avidin that had not been reacted.After 6 h, the product was collected by centrifuging at 17,000×g for 4 min and rinsing three times with deionized water.The BP Avi product was finally stored at 4 °C.

Synthesis of icaritin-biotin (ICT Bio )
Biotin (0.36 g, 1.50 mmol), icaritin (0.15 g, 1.24 mmol), HoBt (0.14 g, 1.00 mmol) and DMAP (0.035 g, Scheme 1 Schematic illustration of the preparation, bone-defect-targeting and multiple bone regeneration mechanisms of M@BP ICT .A The preparation of M@BP ICT .B Multiple bone regeneration mechanisms of M@BP ICT .C The in vivo bone regeneration activity of M@BP ICT 0.10 mmol) were dissolved in 10 mL of DMF.The mixture was stirred for 1 h at room temperature before being cooled to 0 °C in an ice bath.DCC (0.21 g, 1.00 mmol) was dissolved in 10 mL of DMF and then dropwisely added to the the above mixture.After removing the ice bath, the reaction was gradually warmed to room temperature and continued for 24 h.The crude mixture was filtered and rinsed with CHCl 3 prior to vacuum-assisted drying.The dried product was resuspended and dissolved in 20 mL absolute ethanol, heated to 80 °C prior to filtration, and the filter cake was rinsed 2 to 3 times with hot ethanol before being dried to yield 0.26 g of white powder biotin-icartin (31.7%).The structure was verified by 1 H NMR (Bruker ECX 500, Germany).

BP-icaritin (BP ICT ) preparation
Briefly, ICT Bio (10 mg) dissolved in DMF (5 mL) was added with BP Avi (10 mg).In addition, the reaction was maintained for 2 h at room temperature, followed by dialysis to remove excess unreacted biotin-icaritin.The product was collected by centrifuging at 17,000×g for 4 min and rinsing three times with deionized water.The final product was then stored at 4 °C.

Macrophage membranes (MMs) collection
MMs were collected according to previous study [30].Briefly, the macrophages were digested with trypsin, centrifuged at 1000 rpm for 5 min, the supernatant was discarded, and the cells were rinsed with PBS.The reagent for cell lysis (pH 7.4) was consisted of Tris (0.01 M), MgCl 2 (1 mM), phenylmethylsulfonyl fluoride (PMSF, 2 mM), sucrose (0.35 M), and DNase (10 g mL −1 ).Cells were incubated with cell lysate buffer, homogenised at 22,000 rpm for 2 min, and centrifuged at 4 °C, 4000 rpm for 10 min.The supernatant was collected and centrifuged at 4 °C, 10,000 rpm for 20 min.The supernatant was then discarded, and the precipitate was collected following three times of washing with the lysate buffer and dialysis to remove various buffer salts.Finally, the purified MMs were storage as a freeze-drying precipitate.

Preparation of M@BP ICT
MMs (2 mg) were re-dissolved in PBS (1 mL) by the vortex.BP ICT (1 mg) was dispersed in 1 mL of PBS in a water bath.To generate M@BP ICT , the MMs solution was slowly dropped into 100 μL of BP ICT solution under ultrasonic conditions for 5 min.The final product was then stored at 4 °C.

Characterization of nanomaterials
FTIR spectra of BP, ICT, ICT Bio , BP Avi and BP ICT were obtained on the Fourier Transform Infrared Spectrometer (FTIR, Thermo Fisher Scientific, USA).Thermogravimetric analysis (TGA, TA Instruments, USA) was performed on a thermos analyzer.Scanning Electron Microscope (SEM, Thermo Fisher Scientific, USA) and Atomic Force Microscope (AFM, Bruker, German) were conducted to analyze the morphology of BP, BP Avi and BP ICT .Elemental Mapping analyses of BP Avi and BP ICT were performed using the Transmission Electron Microscope/Energy dispersive X-Ray Spectroscopy (TEM-EDS, FEI talos F200x G2, USA).The size distribution of BP, BP Avi , BP ICT and M@BP ICT were characterized by Dynamic Light Scattering (DLS, Wyatt Technology, USA) and Transmission Electron Microscope (TEM, FEI Philips Tecnai 20, USA).The Zeta potential of BP, ICT Bio , BP Avi and BP ICT were obtained by DLS.

Characterization of membrane proteins
MMs and M@BP ICT (at the same MMs concentration of 0.5 mg mL −1 ) were fractionated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).Protein image was obtained after coomassie brilliant blue staining.The cell membrane surface proteins, including interleukin-6 receptor (IL6-R) and tumor necrosis factor receptor type 1 (TNF-R) were detected by western blot assay.

Evaluation of cytokine neutralization
MMs (1 mg mL −1 ) in different polarization states (M0, M1 and M2) were utilized to encapsulate the BP ICT and t were incubated with PBS containing 10% FBS and TNF-α (1000 pg mL −1 ) or IL-6 (1000 pg mL −1 ) at 37 °C for 1 h.Samples were then centrifuged at 10,000 rpm at 4 °C to remove the nanomaterials, and the concentration of cytokines in the supernatant were measured by ELISA kits (Multi Sciences, China).

In vitro photothermal measurement
The temperature changes of BP ICT and M@BP ICT upon NIR irradiation were monitored using a visual thermal imaging camera (FLIR, USA).Briefly, 300 μL of BP ICT or M@BP ICT suspension (2.0 mg mL −1 BP ICT ) was respectively exposed to NIR irradiation (808 nm, 1.0 W cm −2 , 10 min).The photo-thermal images were captured continuously at various time intervals.

Photothermal conversion efficiency measurement
The photothermal conversion efficiency can be used to evaluate the photothermal conversion performance of nanomaterials.By continuously heating a suspension of water at a certain concentration under laser irradiation until a stable temperature is reached, then switching off the laser and recording the temperature-time curve during the cooling process.The photothermal conversion efficiency can be calculated based on this cooling curve using a specific formula according to the literature [31].

In vitro release of ICT
Under various pH conditions, the ICT released from M@BP ICT was investigated in PBS with or without NIR irradiation (808 nm, 1.0 W cm −2 ).Two copies of M@ BP ICT were placed in dialysis tubes (MWCO: 3500) and immersed in 20 mL of release medium with different pH conditions (pH 7.4 and pH 4.5) while stirring at 37 °C.M@BP ICT samples were irradiated for 10 min by NIR at predetermined intervals, and then returned back into the dialysis tube and immersed into the release medium.At the specified intervals, 1 mL of the release medium was collected and replaced with new medium.The amount of released ICT was measured by the High Performance Liquid Chromatography (HPLC, Agilent Technologies Co. USA) and calculated according to an ICT standard curve.Release experiments were conducted three times, and the results were presented as the mean standard deviation (SD).

In vitro degradation of BP ICT
The different formulations of BP (1 mg) was placed in an EP tube containing 1.5 mL of SBF.The NIR irradiation was performed following condition: 808 nm at 1.0 cm −2 , 10 min per day, and the other groups without NIR irradiation were placed at room temperature for 7 days.Elemental mapping analysis (C, O, and Ca) were carried out on the elemental analyser, and the presence of Ca 3 (PO 4 ) 2 was analyzed by X-ray diffraction (XRD, Shimadzu, Japan).Experiments were performed according to experimental methods in the literature [32].The release of PO 4 3− from BP ICT was determined using a phosphate assay kit (ab65622, Abcam, Cambridge, UK) to measure the concentration of PO 4 3− in the double-distilled water (ddH 2 O).The absorbance at 650 nm wavelength was read on a UV-Vis absorbance microplate reader (Thermo Fisher Scientific, USA), and the concentration of PO 4 3− was determined using the standard curve method.10 mL BP ICT solution (1486.8 μg L −1 , calculated by 1 μM ICT) was placed in an EP tube, and before each measurement, it was centrifuged, and 10 mL of the supernatant was taken for PO 4 3− content measurement.Subsequently, 10 mL of ddH 2 O was added to the original EP tube to continue immersing BP ICT in a 37 °C constant temperature chamber.When calculating the degradation rate, the total PO 4 3− content was quantified based on the total amount of BP ICT added to the test tube.Additionally, the BP ICT +NIR group required additional NIR irradiation (808 nm, 1.0 W cm −2 , 10 min per day).

Cells and cell culture
BMSCs and Raw 264.7 were purchased from National Collection of Authenticated Cell Cultures (Shanghai, China).BMSCs were cultured in α-MEM complete medium (10% FBS, 1% penicillin-streptomycin solution), and RAW264.7 cells were in H-DMEM complete medium (10% FBS, 1% penicillin-streptomycin solution).Cells were incubated in a constant temperature containing 5% CO 2 incubator at 37 °C (Shanghai Jing Hong Co, China).Medium was changed every 2 days.Cells were passaged upon 80-90% confluence under the microscope (Olympus, Japan), and P3 generation cells were obtained for subsequent experiments.

In vitro cellular uptake assays
BMSCs (2.5 × 10 4 cells/well) were seeded in a 24-well plate with fibronectin-coated glass slides.After 12 h incubation, the cells were incubated with Cy5.5-modified M@BP ICT (BP ICT : 500 μg mL −1 , calculated by BP) for different time.The Cy5.5-modified M@BP ICT was prepared by the reaction of the NHS-ester on Cy5.5 with the hydroxyl group of the ICT on BP ICT .Briefly, Cy5.5-NHS (2 mg, dissolved in 1 mL DMSO) was added slowly dropwise to BP ICT (50 mg, dispersed in 5 mL PBS), and reacted at room temperature in the dark for 6 h.After that, unreacted Cy5.5-NHS was removed by dialysis in deionized water.The culture medium was discarded and the cells were rinsed twice with PBS and then fixed with 4% paraformaldehyde for 15 min.DAPI (5 μg mL −1 ) was used to stain the cell nucleus at room temperature for 10 min.The slides were scanned with CLSM and analyzed by the ZEN software (ZEISS, Germany).The cellular uptake of nanoparticles was further quantified by flow cytometry (FCM).Briefly, BMSCs were cultured in a 24-well plate (1.0 × 10 5 cells/well) overnight followed by the treatments as mentioned above.After discarding the culture medium, the cells were rinsed thrice with PBS and treated with trypsin.The cell suspensions were washed twice with PBS, re-suspended in PBS and analyzed by FCM.

Cytotoxicity analysis
Cell proliferation was measured with CCK8 assay.Briefly, BMSCs were seeded and co-cultured with different nanomaterials in 96-well plates at a density of 3 × 10 3 cells/ well.At 24 h, 48 h, 72 h and 120 h, 10 μL of CCK8 reagent (100 μL medium/well) was added and incubated with cells for 2 h at 37 °C, 5% CO 2 .The absorbance of each well at 450 nm was measured with an automatic enzyme instrument (Bio Tek, USA).Live/Dead staining was conducted according to the manufacturer's instructions after cells were cultured as above.Finally, dead cells were stained red, and live cells were stained green.Fluorescence images were captured using an inverted fluorescence microscope (Olympus, Japan).

Cell scratch test
The effects of the various nanomaterials on cell migration in vitro were evaluated using a wound healing assay.BMSCs were cultured in 6-well plates until they attained at least 90% confluence for the scratch tests.A 200 μL sterile pipette tip was used to scratch the BMSCs monolayer after starvation for 24 h.Subsequently, the original medium was replaced by BP ICT or M@BP ICT complete medium.Finally, an inverted microscope (Olympus, Japan) was used to record the migration positions at 24 h.The migration area was calculated using ImageJ software (NIH, Bethesda, MD, USA).

Osteogenic induction under inflammatory-free microenvironment
The BMSCs were seeded at 2.5 × 10 4 cells per well in 12-well plates in the complete medium for 24 h.Then replacing the original medium with osteogenic induction medium (50 μg mL −1 ascorbic acid, 10 mM β-glycerol Sodium phosphate and 100 nM dexamethasone were added into the complete medium) with the presence of BP Avi , BP ICT and M@BP ICT , respectively and cultured for 14 and 21 days.Typically, one group was cultured without the NIR irradiation.The other group was irradiated with NIR, 10 min per day.Osteogenic induction medium was changed every 2 days.ALP and alizarin red S (ARS) staining was then performed at days 14 and 21 of osteogenic induction, respectively.Furthermore, western blotting was conducted at 7 days of osteogenic induction.

Osteogenic induction under inflammatory microenvironment
The osteogenic induction medium under inflammatory microenvironment was prepared according to the literature [34]: Raw 264.7 cells were seeded in 12-well plates at a density of 5 × 10 5 per well.The culture medium was collected from the control, LPS, LPS+BP ICT , LPS+M@ BP ICT and LPS+M@BP ICT +NIR after 24 h co-cultured.
After centrifugation, the supernatant was mixed at a 1:2 ratio by volume with osteogenic induction medium to obtain osteogenic induction medium under inflammatory microenvironment.According to the different types of Raw 264.7 culture medium in configuring the osteogenic induction medium under inflammatory microenvironment, it was divided into the following groups: control, LPS, LPS+BP ICT , LPS+M@BP ICT, and LPS+M@ BP ICT +NIR groups.The rest of the steps were as described above.After 14 days of osteogenic induction, ALP staining was conducted to evaluate the osteogenic differentiation level.

ALP and ARS staining
For each predetermined point, ALP staining was used to assess osteogenesis capacity of each group.Briefly, the supernatant was removed and the cells were rinsed with PBS followed by the addition of 4% paraformaldehyde for 5 min, then rinsed with distilled water.Next, the cells were incubated with ALP staining solution for 20 min.The blue areas in an inverted fluorescence microscope could be interpreted as positive areas.The pre-processing steps of ARS staining were described as above, and calcium nodule formation can be seen as orange-red in an inverted fluorescence microscope (Olympus, Japan) and was interpreted as a positive area using Image J software.

Flow cytometry
An analysis of the abundance of the M1 marker CD86 was carried out by flow cytometry.Following 48 h of culture, cells were collected, washed, blocked for 15 min with blocking buffer, and then stained for 1 h with PEconjugated anti-CD86 antibodies.Then the cells were centrifuged to remove extra antibodies and rinsed with PBS.Finally, the cells were analyzed using a flow cytometer and analyzed with FlowJo software.

ELISA
Raw 264.7 cells were seeded in 12-well plates at a density of 5 × 10 5 per well.The culture medium was collected from the control, LPS, LPS+BP ICT , LPS+M@BP ICT and LPS+M@BP ICT +NIR after 48 h co-cultured.After centrifugation, the supernatant was collected.Cytokines (TNF-α and IL-6) in the supernatant were measured using an ELISA kit according to the manufacturer's instructions.

Western blotting
After osteogenic induction for 7 days, cells were collected and lysed in RIPA buffer.BCA protein assay kit was used to measure the concentration of protein samples.Then, proteins were separated by SDS-PAGE and transferred to PVDF membrane.The membrane was blocked with rapid blocking solution for 15 min before being incubated with relevant antibodies (BMP-2, Runx2, ALP and β-Actin), followed by the second antibody.Finally, the target protein fragment was detected using a chemiluminescence image analyzer (BIO-RAD, USA), and the grey scale of protein bands was quantified by Image J software.

RNA-seq
Cell lysis was performed using Trizol reagent, and the cell lysate was stored at − 80 °C prior to sequencing.The Illumina Novaseq ™ 6000 platform (LC Bio Technology CO., Ltd., Hangzhou, China) was used for paired-end sequencing with a sequencing mode of PE150.Subsequently, the sequencing data was aligned to the human genome (Homo sapiens, GRCh38) using HISAT2 software (https:// ccb.jhu.edu/ softw are/ hisat2).Assembly of genes or transcripts and FPKM quantification were performed using StringTie software (https:// ccb.jhu.edu/ softw are/ hisat2).Differential analysis was carried out using the R package edgeR (https:// bioco nduct or.org/ packa ges/ relea se/ bioc/ html/ edgeR.html), with a fold change > 2 and a p-value < 0.05.Finally, GO and KEGG pathway enrichment analysis were performed using DAVID software (https:// david.ncifc rf.gov/).The RNA sequencing and analysis were conducted with the assistance of LC Bio Technology CO., Ltd.

qPCR
Total RNA was extracted from the cells and reverse transcribed into cDNA for real-time qPCR analysis.The primers for the target genes were synthesized by Wuhan Servicebio Technology Co., Ltd, and all the sequences are shown in Additional file 1: Table S1. 2 −ΔΔCт method was used to normalize the gene expression.

Animal handing
The study was approved by the Ethics Committee of Affiliated Hospital of Nanjing University of Chinese Medicine (Approval No: 2021 DW-11-02).Six-week-old male C57BL/6 mice weighing 19-21 g were randomly divided into six groups (n = 12).All mice received humane care according to the guideline of the Guidebook for the Care and Use of Laboratory Animals.A traditional murine cranium defects model was applied in this study [35].First, the mice were anesthetized with isoflurane.A non-critical sized circular defect (Φ3 mm with full-thickness) was then made on the cranium of each mouse by a trephine.The bone defects repair experiments were conducted by injecting different nanomaterials through the tail vein, and PBS group was used as control.After the mice were sacrificed at week 2 and week 4, the craniums and major organs (heart, liver, spleen, lung and kidney) were harvested.Part of the craniums were further immersed in liquid nitrogen, and the rest samples were stored in the paraformaldehyde solution.
Unless otherwise specified, the following dosages calculated by 8 mg kg −1 of ICT were adopted in the animal experiments: PBS (100 μL), M@BP Avi (24.3 mg kg −1 ), M@ BP ICT (32.3 mg kg −1 ), M@BP ICT (32.3 mg kg −1 ).Each group of mice with bone defects received a tail vein injection of 100 μL of the formulations once a week.For the groups involving NIR irradiation, the bone defects area was irradiated with NIR irradiation (808 nm, 1.0 W cm −2 , 10 min) under anesthesia 6 h after the administration.

In vivo biodistribution
In vivo biodistribution of M@BP ICT-Cy5.5 was demonstrated using the C57BL/6 mice bearing cranium injury.The mouse with cranium injury model was established by drilling hole with a handheld grinding drill.After 3 days, the skull injury mice were randomly grouped and injected with a single dose of Cy5.5-modified BP ICT and M@BP ICT (BP ICT : 32.29 mg kg −1 ) in 100 μL of PBS via tail vein.Cy5.5-modified BP ICT was prepared by the reaction of the NHS-ester on Cy5.5 with the hydroxyl group of the Tris on ICT.Briefly, Cy5.5-NHS (2 mg, dissolved in 1 mL DMSO) was slowly dropped into BP ICT (50 mg, dispersed in 5 mL PBS), and reacted at room temperature in the dark for 6 h.After that, unreacted Cy5.5-NHS was removed by dialysis in deionized water.At designated time intervals (0, 1, 2, 4, 8, 12 h) post-injection, the fluorescence images were acquired.At the end of experiments, the major organs were dissociated after euthanasia for ex vivo fluorescence imaging.The fluorescence images were scanned using a near-infrared fluorescence imaging system at an excitation of 675 nm and emission of 694 nm, and the images were acquired and analyzed using the Living Image software (PerkinElmer, USA).

In vivo photothermal measurement
The mice received intravenous administration of PBS, BP ICT and M@BP ICT respectively.Six hours later, the cranium defects area in the mice under anesthesia was irradiated with NIR irradiation (808 nm, 1.0 W cm −2 , 15 min).A thermal imaging camera (FLIR, USA) was utilized to capture the photo-thermal images and the corresponding temperature variation was monitored.

Micro-CT
The new bone formation and bone quality of cranium defects area were evaluated by micro-CT (Hiscan, China).The scan parameters were set as 80 kV, 500 μA current, and 25.0 μm pixels.After 3D reconstruction, bone mineral density (BMD), trabecular number (Tb.N), trabecular thickness (Tb.Th) and bone tissue volume/ total tissue volume (BV/TV) were measured and recoded by Hiscan Analyzer software (V3.0).

Histological staining
Craniums were completely decalcified in 10% EDTA and dehydrated in a graded ethanol series, and then embedded in paraffin.The specimens were cut into 5 μm thick sections for histological and immunohistochemical analysis.According to the instructions, H&E, Goldner, BMP-2, CD31, IL-10 and TNF-α staining were performed.An optical microscope (Nikon, Japan) was used to analyze the results.Image J software was used to assess the integrated optical density (IOD) value of the positive area.

In vivo biosafety analysis
Major organs, including hearts, livers, spleens, lungs, and kidneys, were excised, fixed with 4% paraformaldehyde and sectioned for H&E staining.Besides, the weight of mice at observed time points (0 d, 2 d, 5 d, 7 d, 14 d, 21 d and 28 d) had been recorded.

Statistical analysis
All data were presented as Mean ± SD.ANOVA with a Tukey post-hoc test was used to conduct data analysis between each group unless indicated otherwise.*P < 0.05, **P < 0.01, ***P < 0.001 or # P < 0.05, ## P < 0.01, ### P < 0.001 were considered significant.Quantifications were done with Image J software on high-resolution images.Statistical analysis was performed using SPSS 22.0 Software (IBM, USA).

Fabrication and characterization of M@BP ICT
The naked BP was prepared by the method of "top-down" liquid-phase mechanical exfoliation [30,36].Then, biotin-coupled ICT was synthesized and confirmed by 1 H NMR before being connected to BP (Additional file 1: Figs.S3 and S4).To facilitate the BP with high loading efficiency for biotin-coupled ICT, avidin was covalently modified to the surface of BP (Additional file 1: Fig. S5), which could serve as a universal and versatile platform for drug delivery.Accordingly, biotin-coupled ICT could be efficiently linked with BP Avi via non-covalent interaction, which was confirmed by FTIR assay (Fig. 1A).As shown in Fig. 1A, the successful modification of BP and avidin was demonstrated by the presence of characteristic peaks in BP Avi , such as the N-H stretching vibration peak at 3312 cm −1 .Additionally, peaks at 1602 and 2930 cm −1 indicated the presence of the phenyl ring and methylene group, respectively.Peaks at 1721 and 1200 cm −1 confirmed the successful coupling of ICT and biotin.The carbon-carbon double bond exhibited a peak at 1667 cm −1 .Detection of these characteristic peaks in BP ICT indicated the ICT was successfully connected to the BP.Additional file 1: Fig. S6 indicated that XRD of BP exhibits prominent characteristic peaks at 16.9°, 26.6°, 34.3°, and 52.4°.The intensities and positions of the diffraction peaks are in basic agreement with BP's crystal database standard card (JCPDS No. 47-1626), corresponding to the (020), (021), (040), and (060) crystal planes of BP.This indicated that the BP prepared by the liquid exfoliation method have high purity and demonstrates the stable and excellent crystal structure of the obtained BP.Furthermore, the diffraction peaks of BP ICT are highly consistent with BP, indicating that the surface modification of the compound does not affect its crystalline characteristics.Furthermore, TGA was performed to calculate the drug-loading capacity of BP.By analyzing the mass difference corresponding to the weight loss stages of BP and ICT on the BP ICT TGA curve, the mass ratio of ICT to BP in BP ICT is determined to be 2:5.(Fig. 1B).The AFM revealed that the thicknesses of BP (Additional file 1: Fig. S7), BP Avi , and BP ICT were 25.1, 32.9, and 49.4 nm, respectively (Fig. 1C) and thickness elevation might be attributed to the surface modification.Furthermore, the SEM results demonstrated that different formulations of BP were well maintained as a nanosheet structure (Additional file 1: Fig. S8).The modification of ICT on BP was confirmed by element mapping demonstrating that the increased carbon and oxygen elements (main elements of ICT) were detected on BP (Fig. 1D).Furthermore, the presence of sulfur and nitrogen elements (specific elements of avidin and biotin) on BP indicated that ICT was connected to BP through the interaction of avidin and biotin.Theoretically, S should not be present on BP Avi , but the little presented S element may be due to the use of S-containing sulfo-SMCC during the synthesis of BP Avi (Additional file 1: Fig. S9).Additionally, due to the successful loading of ICT on the material, the C and O elements in BP ICT have increased by 9.2% and 3.49% respectively compared to BP Avi .The above studies demonstrated the ICT was successfully coupled with BP to form BP ICT through the non-covalent conjunction of avidin and biotin.The acute phase of bone defects is always accompanied with the secretion of excessive pro-inflammatory factors, which can significantly inhibit the osteogenic differentiation of BMSCs [37].Instead, inflammatory microenvironments could be targeted by inflammatory cells or the derived membrane.The cell membranes extracted from macrophages were employed to encapsulate the BP ICT to endow it with bone-defect-targeting ability.Previous research indicates that small-sized BP (with a mean diameter of 208.5 ± 46.9 nm) demonstrates better biocompatibility [38].Subsequently, the size and morphology were investigated by DLS and TEM.As shown in Fig. 1E, F, the average diameter of BP ICT and MMs-encapsulated BP ICT (M@BP ICT ) was about 174 nm and 198 nm, respectively, which was significantly larger than naked or avidinmodified BP (Additional file 1: Fig. S10).Therefore, these nanomaterials could exhibit good biocompatibility.Additional file 1: Fig. S11 demonstrated that due to the presence of oxidized phosphoric groups on the surface of BP, the surface potential of exposed BP is relatively negative at − 37.9 mV.Following the binding of the avidin, the surface potential increases to − 27 mV due to the positively charged avidin neutralizing some of the negative charges under neutral pH conditions.Subsequently, upon binding with ICT Bio , the surface potential further increases to − 20.6 mV.The negative charge is advantageous for the long circulation of biomimetic BP nanocomposites in vivo, avoiding clearance by the body's immune system, particularly the reticuloendothelial system.Furthermore, M@BP ICT were characterized with obvious bilayer membrane structure revealed by the TEM image.The similarity of Coomassie brilliant blue staining indicated that the membrane proteins of the MMs were retained in ultrasound-prepared M@BP ICT (Fig. 1G).Moreover, western blot assay demonstrated that IL-6R and TNF-R protein could be detected in both MMs and M@BP ICT (Fig. 1H), indicating that M@BP ICT might decrease inflammatory reaction by adsorbing pro-inflammatory factors in the bone defects area.

Evaluation of photothermal property and degradation processes
The MMs inherited the membrane from macrophages, which might be advantageous to the adsorption of inflammatory factors.Therefore, the adsorption capacity of M@BP ICT for inflammatory cytokines was evaluated  2A, both of the membranes from M0 and M1 macrophages could absorb inflammatory factors of IL-6 and TNF-α through the cytokine receptors on the membrane.In contrast, the cell membranes from the anti-inflammatory M2 macrophages did not exhibit significant absorbed effect.Furthermore, the photothermal performance of M@BP ICT was studied by monitoring the temperature variation upon different period of NIR irradiation.The results demonstrated the photothermal characteristic of BP was well maintained after the chemical modification and MMs encapsulation indicated by the elevated temperature as the NIRexposure time extended.The temperature of M@BP ICT could reach up to 46.4 °C after 10 min NIR irradiation (Fig. 2B, C).BP could serve as a stable and efficient drug delivery system under the chemical modification and interaction of biotin and avidin.Previous studies have demonstrated that the cargos delivered by BP could be released in response to acid microenvironments and NIR irradiation since both of the conditions could accelerate the degradation of BP [36].According to the published method [31], under 808 nm laser irradiation, BP ICT and M@BP ICT demonstrated a photothermal conversion efficiency (η) of 27.3% and 26.1% respectively (Additional file 1: Fig. S12).Next, the release of ICT from BP under different pH conditions and NIR exposure was studied.As shown in Fig. 2D, the acid condition or NIR exposure could accelerate the ICT release from BP compared with the condition of pH7.4.However, the combination of NIR and lower pH value exhibited the intense effect on ICT release and about 77.34% of ICT was released after three times of NIR irradiation in pH4.5.The acidic microenvironment is one of the characteristics of the bone defect microenvironments, which can lead to the inhibition of osteoblast activity, the increase of osteoclast activity, and the inhibition of bone formation [39,40].The release of PO 4 3− from the degradation of BP ICT gradually increased with time, indicating that BP ICT has good degradability (Additional file 1: Fig. S13).During the observing period, the release of PO 4 3− in the BP ICT +NIR group remains consistently higher compared to the BP ICT group.This indicated that NIR irradiation can enhance the degradation of BP ICT , promoting the release of PO 4 3− .Thus, ICT and PO 4 3− will be rapidly released from BP-based nanomaterials in the bone defects microenvironment upon NIR irradiation, which would be advantageous to the regeneration of bone defect.Accordingly, the Ca element recruitment was examined on the surface of BP by TEM elemental mapping analysis.As shown in Fig. 2E and Additional file 1: Fig. S14, Ca element could be detected after the BP were incubated with SBF for 7 days.However, upon NIR irradiation, more Ca elements were recruited on the surface of BP and could reach up to 6.23% indicating that NIR exposure could accelerate the degradation of BP facilitating the recruitment of Ca element for bone repair.The results were confirmed by XRD demonstrating that the diffraction peaks of Ca 3 (PO 4 ) 2 (2θ = 20.074,27.288, 31.507,45.157) could be observed in the BP ICT +NIR group (Fig. 2F).In contrast, only the diffraction peaks of BP (2θ = 16.813,26.451, 34.067, 52.176, 55.907) were observed in the control group (BP ICT 0d).Similarly, in the room-temperature group (BP ICT 7d), only the crystalline phases of BP changed (2θ = 16.736,26.295, 33.921, 51.943, 55.596).These above results indicated that M@BP ICT can absorb inflammatory factors and simultaneously provide bone repair factor and material for bone repair, achieving a one-step method for promoting osteogenesis.In addition, the microenvironment of bone defects is conducive to the degradation of BP ICT , which could facilitate the release of ICT and the formation of Ca 3 (PO 4 ) 2 .Moreover, PTT derived from NIR irradiation could expedite this procedure and facilitate mineralization.

Co-culture study of nanomaterials and BMSCs
Since M@BP ICT might promote the bone regeneration, the endocytosis and biocompatibility were firstly evaluated on BMSCs, which could differentiate into osteoblast for the bone formation [41].It was interesting to notice that the introduction of MMs could increase the endocytosis for BP ICT indicated by the stronger fluorescence that nearly all of BMSCs endocytosed the Cy5.5-modifiedM@BP ICT after 6 h of incubation.(Fig. 3A).Our previous research indicated that the drug-loaded BP encapsulated within MMs were almost entirely engulfed by cells.Therefore, when M@BP ICT reaches the site of bone defects area, M@BP ICT maybe uptake by cells via caveolae-dependent endocytosis [36].Furthermore, the M@ BP ICT exhibited a prominent biocompatibility that the BMSCs were maintained alive indicated by the green fluorescence after being incubated with M@BP ICT for 120 h (Fig. 3B, C).The NIR irradiation also did not show cytotoxicity to the BMSCs, which might be attributed to the moderated photothermal effect and the temperature was maintained below 42 °C.ICT could promote the migration of BMSCs via the MAPK signaling pathway to facilitate osteogenic differentiation at bone defect area [42,43].Therefore, whether the biotin-modification or loaded on BP would influence the activity of ICT on BMSCs were examined by the cell scratch assay.As shown in Fig. 3D, E, free ICT and M@BP ICT could significantly promote the migration of BMSCs compared with PBS group.However, the M@BP ICT induce more migration than the non-modified ICT, which might be contributed from the enhanced cellular uptake of the nanoparticles.The studies demonstrated that MMs-encapsulated BP ICT with good biocompatibility could promote endocytosis and migration for BMSCs, thereby initiating and accelerating the process of bone regeneration.

In vitro osteogenesis under inflammatory microenvironment
The acute phase of bone injury is usually accompanied by a large accumulation of pro-inflammatory factors, and pro-inflammatory factors (TNF-α, IL-6) could enhance osteoclast activity and impair bone regeneration.Therefore, regulation of inflammatory factor levels is a crucial method for promoting early-stage bone regeneration.To this aim, we evaluated whether M@BP ICT could promote osteogenesis in the inflammatory microenvironment by simulating in vitro the early inflammatory microenvironment following bone defects in vivo (Fig. 4A) [34].As shown in Fig. 4B, C, the ALP staining demonstrated that the osteogenic differentiation of BMSCs was significantly decreased by the treatment of LPS.In contrast, BP ICT treatments could reverse LPS-induced reduction of osteogenic differentiation.Furthermore, M@BP ICT exhibited stronger activity on the osteogenic differentiation indicated by the ALP staining, which could be enhanced by the NIR irradiation.The combined effect on osteogenic differentiation might be contributed the inflammatory factor absorption, anti-inflammatory activity, and enhanced mineralization.Accordingly, the inflammatory inhibition mediated by M@BP ICT was examined by measuring the polarization of M0 macrophages.As shown in Fig. 4D, E, BP ICT treatments could significantly decrease the proportion of LPS-stimulated M1 macrophages (CD86-positive).The anti-inflammatory activity could be originated from the ICT-mediated the regulation of inflammatory cytokines and phosphorylation of p38 and JNK [44,45].Consistent with previous results, MMsencapsulated BP ICT could further reduce the ratio of M1 macrophages, which could be significantly strengthened upon NIR exposure.The anti-inflammatory activity of M@BP ICT was confirmed by determining the cytokines secreted from LPS-incubated macrophages.The combination of M@BP ICT and NIR exposure exhibited the strongest inhibition on the expression of inflammatory factors including IL-6 and TNF-α (Fig. 4F).The results indicated that M@BP ICT could inhibit the polarization of M1 macrophages and expression of inflammatory factors, which could enhance osteoclast activity and inhibit osteoblast activity, thereby inhibiting bone repair [46].Consequently, the application of M@BP ICT upon NIR irradiation might take full advantage of the inflammatory microenvironment to enable targeted delivery of drugs in the bone defects area.Subsequently, M@BP ICT could improve the inhibition of osteogenic differentiation by inflammation through modulation of microenvironmental inflammatory factors.

In vitro osteogenesis under inflammatory-free microenvironment
As bone repair progresses, the inflammatory microenvironment tends toward homeostasis.Therefore, the combined effect of M@BP ICT and NIR on in vitro osteogenesis differentiation of BMSCs were examined under Inflammatory-free Microenvironment.The activity of ALP, a typical marker for the early stage of osteoblast differentiation was stained to analyze the activity of M@ BP ICT .As shown in Fig. 5A, B, M@BP ICT treatments are more conducive to the osteogenic differentiation of BMSCs compared with PBS or avidin-connected BP after 14 days of osteogenesis induction.More importantly, the application of NIR irradiation could significantly improve the osteogenic differentiation of BMSCs treated with BP-based nanomaterials, indicating that moderate PTT is an effective osteogenesis strategy.The degradation of BP was advantageous during the process of bone repair.The ARS staining was performed to evaluate the degree of mineralization in BMSCs with different treatments.As shown in Fig. 5C, D, after 21 days of osteogenic induction, relatively few and small mineralized nodules were detected in the control group, whereas abundant mineralized nodules were observed in the BP-treated groups.Except for the control group, more mineralized nodules could be detected in the NIR-irradiated groups than the group without NIR exposure.The reinforced effect of mineralization might be a result of PTT-mediated osteogenesis promotion and the formation of Ca 3 (PO 4 ) 2 from BP degradation.Furthermore, the results were confirmed by the examining the expression of osteogenesis-related proteins demonstrating that M@BP ICT exhibited the strongest activity on the induction of BMP-2, Runx2, and ALP, which could be significantly increased by NIR irradiation (Fig. 5E, F).Overall, the M@BP ICT +NIR group exhibited greater osteogenic potential in vitro than other groups.

RNA sequencing (RNA-seq) analysis of the BP ICT for osteogenic differentiation
To further investigate the molecular mechanisms of BP ICT on the osteogenic differentiation and mineralization of BMSCs, we performed RNA-seq analysis on BMSCs cultured with BP ICT intervention.Figure 6A presented the volcano plot of differentially expressed genes (DEGs), where gray indicates genes with non-significant differences, red and blue represent genes with significantly altered expression.Among the DEGs, 5489 genes were upregulated (red), and 3192 genes were downregulated (blue).Based on the top 100 DEGs shown in the heatmap (Fig. 6B), when compared to the control group, significant upregulation was observed in mitochondrial ribosomal protein L49 (Mrpl49), inhibin subunit beta A gene (Inhba), and thioredoxin-interacting protein (Txnip).Figure 6C presented the results of the Gene Ontology (GO) analysis, indicating that BP ICT can exert osteogenic effects through cell differentiation, positive regulation of cell population proliferation, and immune system processes.Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was conducted (Fig. 6D).The enrichment level was shown on the horizontal axis, with larger bubbles indicating more DEGs.The calcium signaling pathway and PI3K-AKT signaling pathway showed high enrichment, suggesting that BP ICT may accelerate osteogenesis through these pathways.The PI3K-AKT signaling pathway may play a role in regulating cell proliferation, bone metabolism, and macrophage polarization.Furthermore, the upregulation of the calcium signaling pathway further supports the involvement of phosphate ions and calcium ions released by BP ICT in promoting bone defect repair.
Subsequently, real-time quantitative polymerase chain reaction (qPCR) analysis was performed to detect and validate the transcriptionally related genes (Mrpl49, Inhba and Txnip in Fig. 6E) identified from the transcriptomic analysis, as well as the osteogenicrelated genes (BMP-2 and Runx2 in Fig. 6E).Mrpl49 is one of the mitochondrial ribosomal proteins involved in the synthesis and function of mitochondrial proteins.Its interaction with the PI3K-AKT pathway and bone metabolism has not been confirmed yet.However, mitochondria serve as the energy powerhouse within cells and are closely associated with cellular metabolism.Therefore, we speculate that Mrpl49 primarily exerts its osteogenic effects through the regulation of mitochondrial function, cell proliferation, and survival.The PI3K-AKT pathway may indirectly impact cell proliferation and survival by influencing Mrpl49.Inhba is an important growth factor belonging to the TGF-β superfamily.It participates in the regulation of cell proliferation, differentiation, and other biological processes.Inhba also plays a role in modulating molecular signaling pathways associated with bone cell proliferation, differentiation, and bone formation.Therefore, the increased expression of Inhba during osteogenesis may contribute to the promotion and protection of bone formation.The PI3K-AKT pathway exhibits significant interaction with Inhba, involving the regulation of Inhba expression, signaling pathway modulation, cell proliferation, and survival [47,48].Txnip is a crucial regulatory protein involved in multiple biological processes, including cellular oxidative stress, glucose metabolism, apoptosis, and inflammation.Some studies have indicated its involvement in the regulation of osteoblasts, particularly in relation to cell proliferation, differentiation, and bone matrix formation.Additionally, Txnip is also associated with the PI3K-AKT pathway, specifically in the context of oxidative stress and apoptosis [49].
BMP-2 and Runx2 can both be activated through the PI3K-AKT signaling pathway [50,51].BMP-2 is a potent osteogenic inducer, and the PI3K-AKT pathway can promote the transcription and expression of BMP-2, thereby influencing osteogenic differentiation and bone formation during the osteogenesis process.Runx2 is a transcription factor that is essential for osteoblast differentiation, and the PI3K-AKT pathway can impact the activity and regulation of Runx2 through various mechanisms.In addition to the qPCR results (Fig. 6E), the elevation of BMP-2 and Runx2 proteins in cellular experiments (Fig. 5E) aligns with the sequencing results.In conclusion, BP ICT can promote cell proliferation, osteogenic differentiation, and osteogenic behavior of bone cells through the PI3K-AKT signaling pathway, while also exerting immunomodulatory effects.

In vivo bone defects targeting ability and photothermal effects
The MMs could enable BP ICT to target the inflammatory microenvironment and release the cargos in responsive acid and NIR exposure.Therefore, the in vivo bonedefect-target ability of M@BP ICT was investigated since the bone defect area was characterized with inflammatory microenvironments.A non-critical defect with a size of 3 mm was established on the murine cranium to simulate the region of post-traumatic bone defects (Fig. 7A) [35].The mouse was performed with intravenous administration of Cy5.5-modified BP ICT and M@ BP ICT and then monitored with In Vivo Imaging Systems (IVIS) at different time intervals.As shown in Fig. 7B, C, the fluorescence around the cranium could be observed after 1 h of injection with the M@BP ICT , whereas no fluorescence signal was detected in the cranium for the non-encapsulated BP ICT .After 12 h of injection, the major organs including brain tissues were separated for ex vivo fluorescence analysis.The results demonstrated that the M@BP ICT could be detected at the brain tissues, whereas the brain with the treatments of BP ICT was absence of fluorescence indicating that the introduction of MMs could help BP ICT to target the bone defects area.Besides, Strong fluorescent signals could be observed in the liver, lung and limbs of the mouse in the M@BP ICT group, which may be related to the distribution characteristics of macrophages in the body [52][53][54][55].More importantly, M@BP ICT was able to mediate the photothermal effect as indicated by the gradually increasing temperature at the cranium defects area upon NIR irradiation monitored with an infrared thermal camera (Fig. 7D).M@BP ICT exhibited a moderate photothermal effect and could reach up to 41.9 °C within 10 min of NIR irradiation (Fig. 7E).Consequently, the temperature of the bone defects area was maintained within 40 to 42 °C Fig. 8 Micro-CT analysis of bone indexes for mice with cranium defects after different treatments.A Representative micro-CT reconstruction images in coronal and sagittal view in 2nd and 4th week for different groups [PBS (I), M@BP Avi (II), M@BP Avi +NIR (I, II), M@ICT (IV), M@BP ICT (V), M@ BP ICT +NIR (VI)].The initial boundary of cranium defects was marked by light blue area.Scale bars: 3 mm.Quantitative analysis of bone regeneration effect after different treatments, including BMD (B), TB.N (C), Tb.Th (D) and BV/TV (E).[NIR irradiation: 808 nm, 1.0 W cm −2 , 10 min, under anesthesia 6 h after administration.Dosages of injection: PBS: 100 μL, M@BP Avi : 24.3 mg kg −1 , M@ICT: 8 mg kg −1 and M@BP ICT : 32.3 mg kg −1 body weight, calculated by 8 mg kg −1 ICT, 100 μL each time, once every week, i. v.All statistical data are represented as mean ± SD (n = 3. ns P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001 compared between different groups in 2W or 4W.### P < 0.001 compared between 2 and 4W in the same group)] in this study since high temperature might cause cell damage and be disadvantageous to the bone regeneration [56].Furthermore, after 4 weeks of continuous treatment with M@BP ICT +NIR, the photothermal effect could still be detected indicating M@BP ICT possessed the ability to durably target bone defects (Additional file 1: Fig. S15).
In conclusion, M@BP ICT exhibited superior ability to target bone defects area and photothermal effect in vivo.

Radiological evaluation of bone regeneration in vivo
The M@BP ICT could efficiently target the bone defect tissues and exhibit photothermal performance.Accordingly, the combination of M@BP ICT and NIR irradiation on bone repair was evaluated on the mouse with noncritical size cranium defects (Fig. 8A).The model mice were randomly divided into 6 groups (n = 6): PBS group, M@BP Avi , M@BP Avi +NIR group, M@ICT group, M@ BP ICT group, M@BP ICT +NIR group.Each group of mice were performed with one intravenous injection of different formulations (100 μL) per week.At the 2nd and 4th weeks postoperatively, the micro-CT scanning and three-dimensional (3D) reconstructions of the cranium defects area were conducted to evaluate the bone repair.As shown in Fig. 8A, the repairing progress of bone defects in the 4th week was superior compared with the 2nd week.At the end of the 2nd week, M@BP Avi , M@ BP ICT , M@ICT, M@BP ICT , and M@BP ICT +NIR all exhibited significant activity on the bone regeneration (light blue circle area) compared with the PBS group, indicating that the self-healing of bone defect in mice was seriously inadequate.In contrast, the combination of M@ BP ICT and NIR exposure exhibited the strongest activity on the bone regeneration as evidenced by the greatest quantity of new bone tissue and the smallest remaining Fig. 9 Bone tissue analysis for the cranium defects to bone regeneration.H&E staining, Goldner staining, immunohistochemistry staining of BMP-2, TNF-α, and IL-10 staining at 2nd week (A) and 4th week (B).The staining images are higher magnifications of the areas within the blue boxes.Red arrow showed new bone.Scale bars are 1000 μm in original H&E staining, 100 μm under high magnification images.Positive areas of Goldner (C), BMP-2 (D), TNF-α (E) and IL-10 (F) staining were measured by Image J software.(NIR irradiation: 808 nm, 1.0 W cm −2 , 10 min, under anesthesia 6 h after administration.Dosages of injection: PBS: 100 μL, M@BP Avi : 24.3 mg kg −1 , M@ICT: 8 mg kg −1 and M@BP ICT : 32.3 mg kg −1 body weight, calculated by 8 mg kg −1 ICT, 100 μL each time, once every week, i.v.All statistical data are represented as mean ± SD (n = 3. ns P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001 compared between different groups in 2W or 4W.NS P > 0.05; # P < 0.05; ## P < 0.01; ### P < 0.001 compared between 2 and 4W in the same group)] bone defects area.The quantitative analysis for the bone parameters positively correlated with the quality of bone repair demonstrated that the M@BP ICT +NIR group exhibited the highest callus mineral bone density (BMD), trabecular number (TB.N), trabecular thickness (Tb.Th) and bone volume per total volume (BV/TV) at both of the time intervals (Fig. 8B-E).At the 4th week, the M@BP Avi group showed higher BMD compared with the M@ICT group, indicating that the increased bone density might be contributed from the bone repair material of M@BP Avi .Furthermore, NIR irradiation further improved the bone repair effect.Overall, multifunctional nanomaterials (M@BP ICT ) had a significantly better bone regeneration effect than nanomaterials delivering only bone repair material (M@BP Avi ) or bone growth factor (M@ICT).Additionally, appropriate PTT could enhance bone regeneration.

Histological evaluation of bone regeneration in vivo
The M@BP ICT could significantly promote the bone repair based on anti-inflammatory activity, which could be further increased by the NIR irradiation.Therefore, the histological staining was performed to confirm the bone-repaired effect and in vivo anti-inflammatory activity.The H&E and Goldner staining demonstrated that the bone defect treated with the combination of M@BP ICT and NIR irradiation had been near completely repaired by new bone tissues on the 4th week (Fig. 9A-C).Furthermore, the immunohistochemical staining and western blot assay demonstrated the expression of BMP-2 was significantly increased in M@BP ICT +NIR group compared with other groups (Fig. 9D and Additional file 1: Fig. S16), which was consistent with bone regeneration.The in vivo anti-inflammatory activity was evaluated by the immunohistochemical staining of inflammatory TNF-α and IL-10.As shown in Fig. 9A, B, E and F, M@ BP ICT +NIR treatments could significantly reduce the expression of pro-inflammatory factors of TNF-α and increase the expression of anti-inflammatory factors of IL-10.In addition, the biocompatibility of M@BP ICT was evaluated by monitoring body weights of the mice with different treatments (Additional file 1: Fig. S17) and H&E staining of the major organs (heart, liver, spleen, lung and kidney) at the end of experiments (Additional file 1: Fig. S18).The results demonstrated the bodyweights in all the groups exhibited a gradual growth tendency without statistical difference and no pathological changes in the major organs.Overall, the combined application of M@ BP ICT and NIR irradiation exhibited the strongest activity for inflammatory inhibition and bone repair without significant systemic toxicity.

Conclusion
In this study, a multifunctional bone defect area targeting nanocarrier delivery system (BTNDS) was engineered for active targeted bone repair treatment in bone defects area.The system was characterized with bone-defecttarget capacity, inflammatory factors absorbing ability, increased mineralization, acid-and NIR-responsive drug release behaviors for the bone repair.The experimental results indicated that the nanomaterials have the appropriate sizes, and the concentrations used are within the safe range.Furthermore, the final degradation products of BP are non-toxic PO 4 3− .Thus, the nanomaterials could exhibit good biocompatibility.The ICT was taken as a modal drug loaded on the BP via the interaction of biotin and avidin, while BP could impart NIR responsiveness to BTNDS and provide PO 4 3− as bone repair material.The combination of M@BP ICT and NIR exposure could exhibit significant in vitro anti-inflammatory activity and elevated mineralization, thereby promoting bone repair.In a murine bone defect model, M@BP ICT could efficiently target and accumulate at the bone defect tissues facilitating the drug release and photothermal performance.Upon the NIR irradiation, M@BP ICT could significantly decrease the inflammatory reaction and accelerate the bone regeneration.The beneficial effects of M@BP ICT mentioned above provide a promising solution for the clinical non-surgical effective delivery of bone repair factors and materials to treat bone defects.Its application prospects lie in the treatment of non-critical bone defects and in patient intolerant to surgical procedures for bone defects.Overall, the engineered M@BP Avi could serve as a multifunctional drug delivery platform for inflammation intervention and provide a pivotal strategy for the management of bone defects.

Fig. 1
Fig. 1 Preparation and characterization of M@BP ICT .A The FTIR spectra of BP, ICT, ICT Bio , BP Avi and BP ICT .B Thermogravimetric analysis of BP, ICT and BP ICT .C The AFM images of BP Avi and BP ICT .D The elemental mapping images of BP Avi and BP ICT .Scale bars: 50 nm.E, F The diameter distribution of BP ICT (E) and M@BP ICT (F) was measured by DLS and TEM.Scale bars: 100 nm.G Protein profiles of macrophage membrane (MMs) and M@BP ICT with Coomassie brilliant blue staining.H Expression of IL6-R and TNF-R in MMs and M@BP ICT by western blot analysis

Fig. 2
Fig. 2 The functional analysis for M@BP ICT .A The inflammatory factors adsorption of the M@BP ICT with different sources of cell membrane (M0, M1 and M2 macrophages).B Photothermal investigation for the different formulations of BP exposed to different time of NIR irradiation (808 nm, 1.0 W cm −2 ).C Temperature measurement for different formulations of BP exposed to varied time of NIR irradiation (808 nm, 1.0 W cm −2 ).BP ICT or M@BP ICT suspension contained BP ICT at a concentration of 1486.8 μg L −1 calculated by 1 μM ICT.D The cumulative release of ICT from BP ICT with or without NIR irradiation (808 nm, 1.0 W cm −2 , 10 min) at different pH conditions.E TEM elemental mapping for BP Avi , BP ICT and BP ICT +NIR in SBF for 7 d.NIR irradiation: 808 nm, 1.0 W cm −2 , 10 min per day.Scale bars: 50 nm.F XRD pattern of BP ICT after incubation in SBF for 0 day and 7 days.NIR irradiation condition: 808 nm, 1.0 W cm −2 ,10 min per day.The black arrows indicated Ca 3 (PO 4 ) 2

Fig. 5
Fig. 5 The in vitro osteogenic activities of M@BP ICT on BMSCs.A The ALP staining images of BMSCs co-cultured with different formulations of BP with or without NIR irradiation (NIR ON or OFF) for 14 days.NIR irradiation: 808 nm, 1.0 W cm −2 , 10 min per day.Scale bars: 200 μm.B Quantitative analysis of the ALP staining.C The ARS staining images of BMSCs co-cultured with different formulations of BP with or without NIR irradiation (NIR ON or OFF) for 21 days.NIR irradiation: 808 nm, 1.0 W cm −2 , 10 min per day.Scale bars: 200 μm.D Quantitative analysis of ARS staining.E Western blot analysis and summarized quantitative data (F) for BMP-2, Runx2, and ALP of BMSCs co-cultured with different formulations of BP with or without NIR irradiation (NIR ON or OFF) for 7 d.NIR irradiation: 808 nm, 1.0 W cm −2 , 10 min per day.[BP Avi : 565.4 μg L −1 , BP ICT : 1486.4 μg L −1 , M@BP ICT : at the same BP ICT concentration of 1486.4 μg L −1 , calculated by 1 μM ICT.All statistical data are represented as mean ± SD (n = 6; **P < 0.01; ***P < 0.001 compared between different groups in NIR ON or NIR OFF.NS P > 0.05; # P < 0.05; ### P < 0.001 compared between NIR ON and NIR OFF in the same group)]

Fig. 7
Fig. 7 In vivo evaluation of bone-defect-targeting ability and photothermal performance.A Schematic illustration of the M@BP ICT -based PTT to accelerate bone regeneration.B In vivo fluorescence images for the biodistribution and dissociated major organs from mice injected with Cy5.5-modified BP ICT with or without MMs encapsulation.C Quantitative analysis of the fluorescence distributed in the major organs.D NIR image and temperature curve (E) for the cranium defects with PBS, BP ICT and M@BP ICT upon different time of irradiation.[NIR irradiation: 808 nm, 1.0 W cm −2 ,10 min each time.Intravenous injection: PBS: 100 μL, BP ICT : 32.3 mg kg −1 and M@BP ICT : 32.3 mg kg −1 body weight, calculated by 8 mg kg −1 ICT, 100 μL each time.All statistical data are represented as mean ± SD (n = 3, ***P < 0.001)]